Human Milk Oligosaccharides

Lacto-N-fucopentaose V (LNFP V)

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The neutral fucosylated HMO Lacto-N-fucopentaose V (LNFP V) occurs with a concentration of 0.03 (± 0.02) g/l in human milk1,2,3. The presence of rare pentasaccharides potentially protects against bacterial infections. It was shown to negatively correlate with intestinal Enterobacteriaceae in preterm infants including pathogens associated with inflammation and sepsis4. In addition, LNFP V binds to toxin A of the diarrhea-causing Clostridium difficile, preventing binding of the pathogen to human receptors5. Higher abundance of commensal bacteria such as Bacteroides species can be correlated to LNFP V in breast milk6.

At Jennewein Biotechnologie, LNFP V is presently in development.
 


References:

  1. Thurl, Stephan, et al. “Systematic review of the concentrations of oligosaccharides in human milk.” Nutrition reviews11 (2017): 920-933.
  2. Bao et al. “Quantification of neutral human milk oligosaccharides by graphitic carbon HPLC with tandem mass spectrometry. ” Biochem. 433.1 (2013): 28–35.
  3. Hong et al. “Label-Free Absolute Quantitation of Oligosaccharides Using Multiple Reaction Monitoring. ” Analytical Chemistry, 86.5 (2014): 2640-2647
  4. Underwood et al. “Human Milk Oligosaccharides in Premature Infants: Absorption, Excretion, and Influence on the Intestinal Microbiota. ” Res. 78.6 (2015): 670-7.
  5. Nguyen et al. “Identification of Oligosaccharides in Human Milk Bound onto the Toxin A Carbohydrate Binding Site of Clostridium difficile. ” J. Microbiol. Biotechnol. 26.4 (2016): 659-665.
  6. Borewicz et al. “The association between breastmilk oligosaccharides and faecal microbiota in healthy breastfed infants at two, six, and twelve weeks of age.” Scientific Reports1 (2020): 4270.